Milo is a town located in Piscataquis County, Maine, within the United States. It sits in the valley of the Piscataquis, Sebec, and Pleasant Rivers in the foothills of the Longfellow Mountains. The community includes the village of Derby and the census-designated place of Milo. Its location near the exact center of the state makes it a gateway to pristine outdoor tourist locations.

The community was first known as Township Number 3 in the seventh range north of the Waldo Patent. It was settled by Benjamin Sargent and his son, Theophilus, from Methuen, Massachusetts, on May 2, 1802. On January 21, 1823, it was incorporated as Milo, named after Milo of Croton, a famous athlete from ancient Croton in Magna Graecia, Italy.

Milo developed into a small mill town with Trafton's Falls providing water power for early industry. The Bangor and Piscataquis Railroad arrived in 1868–1869, leading to the development of Milo Junction as the company town of Derby. This area featured the second largest railroad car shop and repair facility in New England, with extensive brick buildings and employee housing arranged in uniform rows.

The town serves as a gateway to hunting, fishing, hiking, boating, and other outdoor activities at locations such as Schoodic, Seboeis, and Sebec Lakes. It provides access to Mount Katahdin and its backcountry in Baxter State Park and the Katahdin Woods and Waters National Monument. The area also includes historic sites like Katahdin Iron Works and Gulf Hagas.

The population was 2,251 at the 2020 census. The region has a humid continental climate with large seasonal temperature differences, featuring warm to hot summers and cold winters. Milo is situated within a few miles of the exact center of the state of Maine, offering access to various recreational opportunities in the surrounding wilderness.